![]() Phase imaging by the transport equation of intensity. Deterministic phase retrieval: a Green’s function solution. Spatial light interference microscopy (SLIM). Fourier phase microscopy for investigation of biological structures and dynamics. Hilbert phase microscopy for investigating fast dynamics in transparent systems. Digital holography for quantitative phase-contrast imaging. Digital image formation from electronically detected holograms. Three-dimensional structure determination of semi-transparent objects from holographic data. Lattice light-sheet microscopy: Imaging molecules to embryos at high spatiotemporal resolution. High-speed in vivo calcium imaging reveals neuronal network activity with near-millisecond precision. Imaging the dynamics of biological processes via fast confocal microscopy and image processing. Quantifying protein densities on cell membranes using super-resolution optical fluctuation imaging. Mapping molecular statistics with balanced super-resolution optical fluctuation imaging (bSOFI). SOFI simulation tool: a software package for simulating and testing super-resolution optical fluctuation imaging. Live-cell multiplane three-dimensional super-resolution optical fluctuation imaging. ![]() SOFI-based 3D superresolution sectioning with a widefield microscope. Fast, background-free, 3D super-resolution optical fluctuation imaging (SOFI). Imaging live-cell dynamics and structure at the single-molecule level. Far-Field Optical Nanoscopy (Springer, 2015). Diffraction-unlimited imaging: from pretty pictures to hard numbers. Vandenberg, W., Leutenegger, M., Lasser, T., Hofkens, J. Imaging intracellular fluorescent proteins at nanometer resolution. Subdiffraction-resolution fluorescence imaging with conventional fluorescent probes. Sub-diffraction-limit imaging by stochastic optical reconstruction microscopy (STORM). The 4D microscope platform unifies the sensitivity and high temporal resolution of phase imaging with the specificity and high spatial resolution of fluorescence microscopy. ![]() This allowed us to not only image live cells in 3D at up to 200 Hz, but also to integrate fluorescence super-resolution optical fluctuation imaging within the same optical instrument. We realized multi-plane imaging using a customized prism for the simultaneous acquisition of eight planes. The non-iterative phase retrieval relies on the acquisition of single images at each z-location and thus enables straightforward 3D phase imaging using a classical microscope. We propose the combination of a novel label-free white light quantitative phase imaging with fluorescence to provide high-speed imaging and spatial super-resolution. However, going ‘beyond the diffraction barrier’ comes at a price, since most far-field super-resolution imaging techniques trade temporal for spatial super-resolution. Aspose.Words Product Solution Aspose.PDF Product Solution Aspose.Cells Product Solution Aspose.Email Product Solution Aspose.Slides Product Solution Aspose.Imaging Product Solution Aspose.BarCode Product Solution Aspose.Diagram Product Solution Aspose.Tasks Product Solution Aspose.OCR Product Solution Aspose.Note Product Solution Aspose.CAD Product Solution Aspose.3D Product Solution Aspose.HTML Product Solution Aspose.GIS Product Solution Aspose.ZIP Product Solution Aspose.Page Product Solution Aspose.PSD Product Solution Aspose.OMR Product Solution Aspose.SVG Product Solution Aspose.Finance Product Solution Aspose.Font Product Solution Aspose.TeX Product Solution Aspose.PUB Product Solution Aspose.Drawing Product Solution Aspose.Audio Product Solution Aspose.Video Product Solution Aspose.Super-resolution fluorescence microscopy provides unprecedented insight into cellular and subcellular structures. ![]()
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